Immune Monitoring (Alternative Technologies)
|Haematology
ACCREDITATION STATUS:
Pilot programme - Not accredited
CLINICAL APPLICATION:
Lymphocyte subset enumeration
PURPOSE OF PROGRAMME:
To assess a laboratory's ability to calculate the absolute count (cells/µL) and percentage of lymphocyte subsets using alternative technologies to traditional flow cytometers.
ANALYTES:
Analyte | Default Unit |
---|---|
paroxysmal nocturnal haemoglobinuria | cells/µL (absolute count) and percentages |
MATERIAL PROVIDED: Stabilised whole blood
DISTRIBUTIONS PER YEAR: Minimum 4; Maximum 6
SAMPLES PER DISTRIBUTION: 2
FREQUENCY OF DISTRIBUTION: Bimonthly as per Trial Schedule (Please note – The Trial Schedule is a guide only and is subject to change)
PROGRAMME OF ANALYSIS:
Results are entered online via the UK NEQAS LI website. Trials are live for 3 weeks. Results can be entered at any time during this period. Under exceptional circumstances late results are accepted at our discretion via email/fax.
DATA ANALYSIS:
Quantitative analysis. Results are scores against the consensus (defined as the robust mean) absolute counts and percentages from results submitted by participants using a single platform approach
PERFORMANCE SCORING:
z scores using robust means and robust SDs with standard limits of +2 and +3 and -2 and -3 as per ISO 13528 A result between 2.0 and -2.0 would be classed as satisfactory. A result between >2.0 and 3.0 or <-2.0 and -3.0 is seen as an 'Action' result, which highlights a potential issue to the laboratory. Two 'Action' results in a period of 3 samples would result in classification as a 'Critical'. A result above 3.0 or below -3.0 is considered to be a 'Critical' result requiring immediate inves
PERFORMANCE MONITORING:
Participant performance is not monitored at this time
PERSISTENT POOR PERFORMANCE:
Participant performance is not monitored at this time
Leucocyte Immunophenotyping
The External Quality Assessment Scheme for Leucocyte Immunophenotyping was established by Professor David Barnett in 1986 at Northern General Hospital, Sheffield. In 1990 the Service became known as the UK National External Quality Assessment Service for Leucocyte Immunophenotyping. UK NEQAS LI is part of the Sheffield Teaching Hospitals NHS Foundation Trust.
Contact Details
- 4th Floor Pegasus House
- 463a Glossop Road
- Sheffield
- S10 2QD
Leucocyte Immunophenotyping
- Telephone: +44 (0) 114 267 3600
- Fax: +44 (0) 114 267 3601
- Email: lip@ukneqas.org.uk
- Web: http://www.ukneqasli.co.uk/