Leukaemia Immunophenotyping (Part 1)
UKAS Schedule of Accreditation, Accredited to ISO/IEC 17043:2010, Provider 7804
Immunophenotyping of leukaemia
PURPOSE OF PROGRAMME:
To assess a laboratory's ability to immunophenotype a leukaemia sample using flow cytometry and/or immunochemistry.
|a range of leukaemia specific cell surface and cytoplasmic antigens||Antigen status in terms of positive/negative and the population size (%) expressing the antigen|
MATERIAL PROVIDED: Stabilised leukaemic blood diluted in stabilised whole blood
DISTRIBUTIONS PER YEAR: Minimum 4; Maximum 6
SAMPLES PER DISTRIBUTION: 1
FREQUENCY OF DISTRIBUTION: Bimonthly as per Trial Schedule (Please note – The Trial Schedule is a guide only and is subject to change)
PROGRAMME OF ANALYSIS:
Results are entered online via the UK NEQAS LI website. Trials are live for 3 weeks. Results can be entered at any time during this period. Under exceptional circumstances late results are accepted at our discretion via email/fax.
Semi quantitative analysis. Positive/negative results of the malignant population are scored against the consensus positive/negative results submitted by participants.
Participants are scored based on their overall immunophenotype for the core antigens (currently CD2, CD3, CD5, CD13, CD19 and CD20) of the malignant population in terms of the positive/negative expression of these antigens within the malignant population. Each trial is awarded a grade of A to G based on antigens within consensus. Grades A to C are classed as satisfactory and grades D to G are classed as critical.
Participant performance is monitored by means of a rolling n+1 sample window where n equals the number of samples per trial issue.
PERSISTENT POOR PERFORMANCE:
Two 'Critical' classifications in a 12 month period are classed as Persistent Unsatisfactory Performance.
The External Quality Assessment Scheme for Leucocyte Immunophenotyping was established by Professor David Barnett in 1986 at Northern General Hospital, Sheffield. In 1990 the Service became known as the UK National External Quality Assessment Service for Leucocyte Immunophenotyping. UK NEQAS LI is part of the Sheffield Teaching Hospitals NHS Foundation Trust.